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Be aware that the side chain has three methylene groups, so that despite the fact that the terminal amino group might be charged below physiological situations, the facet chain does have significant hydrophobic character. Incubate the membrane for 20 min after which wash with DMF to remove uncoupled amino acids. Immunofluorescent staining of Human melanoma cells, using Rabbit polyclonal to acetyl Lysine (ab21623) at 1:100 dilution.
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